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Updated: Dec 24, 2025

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Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
Published on: August 4, 2009
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A real-time fluorescence turn-on assay for trypsin based on a conjugated polyelectrolyte
Renxuan Liu1, Ying Tan, Cunlong Zhang
1The Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology (Ministry of Education), Department of Chemistry, Tsinghua University, Beijing 100084, P. R. China.
Journal of Materials Chemistry. B
|April 9, 2020
Abstract:
We report a continuous and sensitive fluorescence turn-on assay for trypsin by using an anionic conjugated polyelectrolyte (PPE-CO2H) and a cationic peptide substrate labelled with p-nitroaniline (RxG-pNA). Applications of the assay in trypsin activity study and high-throughput screening of protease inhibitors were demonstrated.

