Intrinsically radiolabeled multifunctional cerium oxide nanoparticles for in vivo studies

Area of Science:

• Nanotechnology
• Biomedical Imaging
• Materials Science

Background:

• Cerium oxide nanoparticles (CONPs) show protective properties, but their in vivo mechanisms are unclear.
• In situ monitoring of CONPs is vital for understanding their biological interactions and properties.
• Developing advanced imaging tools is crucial for tracking nanoparticles within biological systems.

Purpose of the Study:

• To create a multifunctional nanoparticle platform for dual-mode imaging (SPECT/OI) of CONPs in vivo.
• To investigate how intrinsic radiolabeling and extrinsic surface functionalization affect CONP properties.
• To evaluate cell viability, uptake, and biodistribution of surface-modified CONPs.

Main Methods:

• Intrinsic radiolabeling of CONPs with radionuclides (e.g., ¹⁴¹Ce).
• Surface functionalization of radiolabeled CONPs (rCONPs) with polymers (Dextran T10, PAA, etc.).
• Preparation of fluorescent CONPs for cell imaging and SPECT/OI for in vivo tracking.

Main Results:

• Surface functionalization modulated cell viability, uptake, and biodistribution of rCONPs.
• PAA-coated rCONPs showed reduced viability at higher concentrations compared to DT10 coatings.
• SPECT imaging of ¹⁴¹Ce-rCONPs demonstrated liver and spleen accumulation over one week.

Conclusions:

• Intrinsic radiolabeling and surface modification are key determinants of CONP biophysical properties.
• The developed platform enables in vivo tracking and imaging of CONPs.
• This approach enhances understanding of CONP behavior for potential biomedical applications.