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Graphene Enclosure of Chemically Fixed Mammalian Cells for Liquid-Phase Electron Microscopy
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Graphene oxide based fluorescent nanocomposites for cellular imaging.

Yang Sheng1, Xiaosheng Tang, Erwin Peng

  • 1Department of Materials Science and Engineering (DMSE), National University of Singapore (NUS), 7 Engineering Drive 1, Singapore 117574. msexuejm@nus.edu.sg.

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We developed fluorescent nanocomposites using graphene oxide (GO) and Zn doped AgInS2 nanoparticles. These stable, non-toxic probes show potential for biomedical imaging and targeting applications.

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Biomedical Engineering

Background:

  • Graphene oxide (GO) is a 2D carbon material with excellent water solubility and low toxicity, making it suitable for biomedical applications.
  • Developing stable and efficient fluorescent probes is crucial for advanced biomedical imaging.

Purpose of the Study:

  • To prepare novel fluorescent nanocomposites by combining graphene oxide (GO) with Zn doped AgInS2 nanoparticles.
  • To evaluate the photoluminescence stability, biocompatibility, and potential for cellular imaging of the synthesized nanocomposites.

Main Methods:

  • Synthesized fluorescent nanocomposites by hybridizing water-soluble GO sheets with Zn doped AgInS2 nanoparticles.
  • Investigated photoluminescence properties, including stability and spectral shifts, over time.
  • Assessed cellular uptake and cytotoxicity using NIH/3T3 cells.
  • Performed in vitro cellular imaging to demonstrate probe efficacy.

Main Results:

  • The photoluminescence of Zn doped AgInS2 nanoparticles was maintained after hybridization with GO, showing no significant emission shift.
  • The nanocomposites exhibited stable photoluminescence intensity for over three months with minimal quenching.
  • PEGylated AIZS-GO nanocomposites demonstrated efficient uptake by NIH/3T3 cells with no observable cytotoxicity.
  • Successful in vitro imaging of NIH/3T3 cells confirmed the potential of the AIZS-GO-PEG nanocomposites as fluorescent probes.

Conclusions:

  • The developed AIZS-GO-PEG nanocomposites offer a stable and biocompatible platform for fluorescent biomedical imaging.
  • These novel nanocomposites show promise as effective fluorescent probes for targeted biomedical applications.