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Updated: Dec 24, 2025
Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry
Published on: March 1, 2013
Highly water-soluble perylenediimide-cored poly(amido amine) vector for efficient gene transfection
Zejun Xu1, Bicheng He, Wei Wei
1State Key Laboratory of Chemical Resource Engineering, Key Laboratory of Carbon Fiber and Functional Polymers, Ministry of Education, Beijing University of Chemical Technology, 100029 Beijing, China. yinmz@mail.buct.edu.cn.
Abstract:
A highly water-soluble perylenediimide-core poly(amido amine) (PDI-PAmAm) with peripheral amine groups has been synthesized. The central PDI chromophore allows optical monitoring of relevant cellular experiments by fluorescence microscopy. The PAmAm shell provides the steric bulk that notably suppresses the aggregation of the central PDI chromophore in aqueous solution. The peripheral amines provide water solubility and positive charges, and also serve as active sites for the further growth of PAmAm. PDI-PAmAm can be rapidly internalized into live cells with high efficacy of gene delivery and low cytotoxicity. Both in vitro and in vivo experiments demonstrate the high gene transfection efficacy of PDI-PAmAm.
