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An upconversion nanoprobe operating in the first biological window.

Qiang Ju1, Xian Chen, Fujin Ai

  • 1Department of Physics and Materials Science, City University of Hong Kong, 83 Tat Chee Avenue, Hong Kong SAR, China.

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|April 9, 2020
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Summary

New core-shell-shell nanoparticles enable sensitive biodetection by utilizing the first biological spectral window for enhanced light penetration. This overcomes limitations of water and hemoglobin absorption in biological samples.

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Area of Science:

  • Nanotechnology
  • Biomedical Optics
  • Materials Science

Background:

  • Upconversion nanoparticles (UCNPs) offer sensitive biodetection with reduced background noise.
  • Biological environments limit UCNP applications due to light absorption by water and hemoglobin, causing shallow penetration and sample damage.

Purpose of the Study:

  • To develop UCNPs operating in the first biological spectral window (650-900 nm) for improved light penetration.
  • To create UCNPs with minimal absorption by biological tissues for safer and deeper imaging.

Main Methods:

  • Synthesized core-shell-shell nanostructures with a small feature size of approximately 30 nm.
  • Utilized 808 nm excitation to achieve far-red emission at 660 nm.
  • Developed the synthesized nanoparticles as optical bioprobes.

Main Results:

  • Achieved photon upconversion in the first biological spectral window, minimizing absorption by water and biological specimens.
  • Demonstrated dominant emission in the far-red (660 nm) spectral region.
  • Successfully used the core-shell-shell nanoparticles for sensitive biodetection even with tissue wrapping.

Conclusions:

  • Core-shell-shell UCNPs are effective for biodetection in the first biological window, overcoming previous penetration depth limitations.
  • The developed nanoparticles provide sensitive detection in complex biological settings, including through tissue.
  • This advancement holds promise for enhanced in vivo bioimaging and diagnostics.