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Organic Solvent-Based Protein Precipitation for Robust Proteome Purification Ahead of Mass Spectrometry

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Alkaline Extraction-Synchronous Ultrafiltration: A Green and Efficient Strategy for Preparing Oilseed Protein

Ying Qian¹, Fangdan Shi¹, Weiping Jin²

¹Key Laboratory of Oilseeds Processing, Ministry of Agriculture and Rural Affairs, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, People's Republic of China.

Journal of Food Science

|June 26, 2026

Summary

This summary is machine-generated.

A new alkaline extraction-ultrafiltration method efficiently prepares high-purity protein isolates from oilseed meals like flaxseed, rapeseed, and walnut, yielding excellent functional properties for plant-based protein applications.

Keywords:

different pH conditions functional properties oilseed meal protein preparation purity ultrafiltration

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Area of Science:

Food Science and Technology
Biochemistry
Protein Chemistry

Background:

Oilseed meals are a valuable byproduct of oil extraction, rich in plant proteins.
Efficient methods for isolating high-purity, functional protein isolates are in demand.
Traditional methods like acidic precipitation can be limiting.

Purpose of the Study:

To develop an efficient alkaline extraction-synchronous ultrafiltration method for preparing protein isolates from rapeseed, flaxseed, and walnut meals.
To characterize the purity, recovery, and functional properties of the obtained protein isolates.
To explore the potential for co-enriching multiple protein fractions.

Main Methods:

Alkaline extraction of protein from oilseed meals.
Synchronous ultrafiltration for protein isolation and purification.
Analysis of protein purity, recovery rates, and functional properties (foaming, gelling).

Main Results:

Protein isolate purity exceeded 81% for all three sources, with flaxseed isolate reaching >95%.
Recovery rates of 52% (walnut) and 68% (flaxseed) were achieved.
The isolates demonstrated excellent foaming capacities and gelling ability at specific pH and concentrations.
The method successfully co-enriched albumin, globulin, and glutelin fractions.

Conclusions:

The developed method provides an efficient and alternative strategy for industrial-scale production of functional plant protein isolates.
Ultrafiltration offers advantages over acidic precipitation for oilseed protein enrichment.
The resulting protein isolates possess desirable functional properties for food applications.