Carborane-β-cyclodextrin complexes as a supramolecular connector for bioactive surfaces.

Area of Science:

• Supramolecular Chemistry
• Surface Science
• Bioconjugation Chemistry

Background:

• Dynamic and controlled bioactive surfaces are crucial for various applications.
• Existing host-guest systems have limitations in affinity and applicability.
• Novel strategies are needed for advanced surface functionalization.

Purpose of the Study:

• To develop a synthetic strategy for carborane-peptide bioconjugates.
• To create monovalent supramolecular functionalization of beta-cyclodextrin coated surfaces.
• To evaluate the bioactivity and cell interaction of the novel supramolecular system.

Main Methods:

• Synthesis of carborane-peptide bioconjugates.
• Formation of beta-cyclodextrin·carborane-cRGD supramolecular surfaces.
• Characterization using Infrared Reflection Absorption Spectroscopy (IR-RAS), Water Contact Angle (WCA), and Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D).
• Assessment of cell adhesion and spreading properties.

Main Results:

• Efficient and high-affinity formation of beta-cyclodextrin·carborane-cRGD surfaces.
• Demonstrated superior performance compared to existing bioactive host-guest surface assemblies.
• Observed significant enhancement in cell adhesion, spreading, elongated morphology, and focal adhesions.

Conclusions:

• The novel supramolecular immobilization strategy is effective for creating bioactive surfaces.
• The developed carborane-peptide bioconjugates enable precise control over surface functionality.
• This approach offers a promising platform for advanced biomaterials and cell-interactive surfaces.