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Updated: Dec 24, 2025

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Photo-induced chemistry for the design of oligonucleotide conjugates and surfaces
Antonina Vigovskaya1, Doris Abt, Ishtiaq Ahmed
1DFG-Centre for Functional Nanostructures, Karlsruhe Institute of Technology (KIT), Wolfgang-Gaede-Str. 1a, 76131 Karlsruhe, Germany.
Abstract:
A photocaged diene is introduced at the 5'-end of oligonucleotides using the H-phosphonate approach. The photoenol-functionalized DNA is subsequently employed for the conjugation to a protein and the spatially controlled immobilization onto surfaces using a light-induced Diels-Alder cycloaddition. Fully functional protein-DNA conjugates and patterned DNA surfaces are obtained under mild irradiation conditions.
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