Multidimensional hybrid conductive nanoplate-based aptasensor for platelet-derived growth factor detection

Area of Science:

• Biomedical Engineering
• Materials Science
• Nanotechnology

Background:

• Rapid and selective disease diagnosis is crucial for effective treatment.
• Biosensors offer a promising approach for detecting disease biomarkers.
• Platelet-derived growth factor (PDGF-BB) is implicated in various diseases.

Purpose of the Study:

• To develop a highly sensitive and selective aptamer-functionalized biosensor for PDGF-BB detection.
• To utilize a multidimensional hybrid conducting-polymer plate (A_MHCPP) based field-effect transistor (FET) sensor.
• To demonstrate the sensor's capability in detecting low concentrations of PDGF-BB.

Main Methods:

• Fabrication of multidimensional hybrid conducting-polymer plates (MHCPPs) on graphene via electrodeposition and vapor deposition polymerization (VDP).
• Immobilization of amine-functionalized PDGF-B binding aptamers onto the carboxylated polypyrrole surface using covalent bonding (-CONH).
• Construction and testing of the aptamer-functionalized FET sensor for PDGF-BB detection.

Main Results:

• The A_MHCPP-based FET sensor demonstrated high sensitivity and selectivity for PDGF-BB.
• The sensor achieved a detection limit as low as 1.78 fM for PDGF-BB.
• Effective detection was achieved in the presence of interfering biomolecules at room temperature.

Conclusions:

• The developed aptamer-functionalized hybrid conducting-polymer plate FET sensor is a highly effective tool for PDGF-BB detection.
• This biosensor platform shows significant potential for rapid and accurate disease diagnostics.
• The sensor's performance at room temperature highlights its practical applicability.