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Published on: March 31, 2022
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Development of hepatic blocks using human adipose tissue-derived stem cells through three-dimensional cell printing
Ji-Seon Lee1, Hyeon Yoon, Dajeong Yoon
1Burn Institute, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul, Korea.
Journal of Materials Chemistry. B
|April 9, 2020
Summary
This study developed 3D-printed liver scaffolds using human adipose stem cells (hASCs) and hepatocyte-like cells (AHLCs). These scaffolds show promise as bio-artificial livers, aiding liver regeneration and improving outcomes in animal models.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Hepatology
Background:
- Liver transplantation is limited by donor scarcity and immune rejection.
- Current bio-artificial livers offer marginal survival benefits.
- Tissue regenerative medicine using stem cells presents a viable alternative.
Purpose of the Study:
- To differentiate human adipose stem cells (hASCs) into hepatocyte-like cells (AHLCs).
- To fabricate 3D-printed liver regenerative hepatic block scaffolds using hASCs and AHLCs.
- To evaluate the safety and efficacy of these scaffolds in a rat model of acute liver failure.
Main Methods:
- hASCs were differentiated into AHLCs.
- A neutralized type I atelo collagen solution was used for 3D cell printing of hepatic blocks.
- Genipin was used as a cross-linking reagent to stabilize the scaffolds.
- Scaffolds were transplanted into SD rats with induced acute liver failure.
Main Results:
- Transplanted hASCs and AHLCs migrated to the portal vein of hepatic lobules within 4 weeks.
- Serum biochemistry normalized in rats treated with hASC scaffolds.
- Increased interleukin-10 expression was observed in the livers of treated rats.
Conclusions:
- 3D-printed hepatic blocks composed of hASCs and AHLCs are safe and effective.
- These scaffolds can facilitate liver regeneration and serve as potential bio-artificial livers.
- This approach addresses the limitations of current liver disease treatments.
