Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Single-Cell Transcriptomic Analysis Reveals γδ T-Cell Infiltration and Presence of Immune Regulatory Molecules in Biliary Tract Cancer Microenvironment.

American journal of clinical oncology·2026
Same author

Triglyceride/HDL-cholesterol ratio as a predictor for treatment-related severe hypertriglyceridemia in children with lymphoid malignancies.

Scientific reports·2026
Same author

Multifaceted role of RSPO2: Epigenetics, immunoregulatory, and therapeutic insights in colorectal cancer.

Biochimica et biophysica acta. Reviews on cancer·2026
Same author

Chiral Fluorescent Carbon Dots as Multi-Phased Sensors for Hg<sup>2+</sup>, Pd<sup>2+</sup>, and Cysteine Enantiomers.

Analytical chemistry·2026
Same author

KIM-1 in Advanced Papillary and Clear Cell Renal Cell Carcinoma.

European urology·2026
Same author

Circulating exhausted CD8+ effector memory cells differentiate immune checkpoint inhibitor-induced liver injury from other acute immune-mediated liver injuries.

Journal for immunotherapy of cancer·2026

Related Experiment Video

Updated: Dec 24, 2025

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
06:47

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

Published on: September 20, 2011

38.0K

Immuno-silent polymer capsules encapsulating nanoparticles for bioimaging applications.

Jaishree Jeyaraman1, Anna Malecka, Poonam Billimoria

  • 1Department of Chemical Engineering, Indian Institute of Technology Kanpur, Kanpur, Uttar Pradesh-208016, India. srisiva@iitk.ac.in.

Journal of Materials Chemistry. B
|April 9, 2020
PubMed
Summary

PEGylated polymer capsules with nanoparticles are non-toxic imaging agents. These capsules do not activate immune cells like dendritic cells (DCs) and macrophages (MOs), showing potential for safe bioimaging applications.

More Related Videos

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
11:28

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Published on: April 28, 2015

10.7K
Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

4.9K

Related Experiment Videos

Last Updated: Dec 24, 2025

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique
06:47

Formulation of Diblock Polymeric Nanoparticles through Nanoprecipitation Technique

Published on: September 20, 2011

38.0K
Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications
11:28

Biofunctionalized Prussian Blue Nanoparticles for Multimodal Molecular Imaging Applications

Published on: April 28, 2015

10.7K
Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes
08:27

Synthesis and Characterization of mRNA-Loaded PolyBeta Aminoesters Nanoparticles for Vaccination Purposes

Published on: August 13, 2021

4.9K

Area of Science:

  • Biomaterials science
  • Nanotechnology
  • Immunology

Background:

  • PEGylated polymer capsules encapsulating various nanoparticles (e.g., rare-earth orthovanadates, iron oxide) show promise as fluorescence, magnetic, and magnetofluorescence imaging agents.
  • Previous studies confirmed the in vitro and in vivo non-toxic nature of these nanoparticle-loaded polymer capsules.
  • Ensuring immunocompatibility is crucial for clinical translation of these novel bioimaging agents.

Purpose of the Study:

  • To investigate the immunocompatibility of PEGylated polymer capsules loaded with nanoparticles.
  • To assess the interaction of these capsules with key immune cells, including dendritic cells (DCs) and macrophages (MOs).
  • To determine if the capsules trigger immune responses or affect immune cell function.

Main Methods:

  • Investigated the internalization of polymer capsules by DCs and MOs in vitro.
  • Assessed the impact of capsule internalization on cell viability.
  • Analyzed the effect of different capsules on cytokine profiles of DCs and MOs, including IL-12 and IL-10 secretion, to evaluate immune cell polarization.

Main Results:

  • Polymer capsules were efficiently internalized by both DCs and MOs without significantly affecting cell viability.
  • No significant changes in cytokine secretion were observed from DCs exposed to the capsules.
  • The capsules did not alter the polarization of M1 or M2 macrophage subsets, as indicated by IL-12/IL-10 balance.

Conclusions:

  • PEGylated polymer capsules loaded with nanoparticles demonstrate excellent immunocompatibility.
  • These capsules do not activate antigen-presenting cells (APCs) and do not impede DC or MO responses to pathogen signals.
  • The findings support the potential of these nanoparticle-loaded capsules as immunologically silent agents for advanced bioimaging applications.