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A Polyaniline-based Sensor of Nucleic Acids
Published on: November 1, 2016
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β-Ni(OH)2 nanosheets: an effective sensing platform for constructing nucleic acid-based optical sensors
Hui Zhou1, Junbin Peng, Xinlan Qiu
1College of Science, Jiangxi Agricultural University, Nanchang 330045, P. R. China. lulimin816@hotmail.com.
Journal of Materials Chemistry. B
|April 9, 2020
Summary
Beta nickel hydroxide (β-Ni(OH)2) nanosheets serve as a novel fluorescent biosensor platform for DNA and miRNA detection. This platform offers sensitive and selective optical sensing with potential applications in clinical diagnostics.
Area of Science:
- Materials Science
- Nanotechnology
- Biochemistry
Background:
- Two-dimensional (2D) transition metal oxyhydroxides are emerging materials for advanced applications.
- Beta nickel hydroxide (β-Ni(OH)2) nanosheets possess unique optical properties suitable for biosensing.
Purpose of the Study:
- To explore β-Ni(OH)2 nanosheets as a fluorescent biosensor platform for bioanalysis.
- To develop a sensitive and selective optical sensor for microRNA (miRNA) detection.
Main Methods:
- Synthesis and characterization of β-Ni(OH)2 nanosheets.
- Investigation of fluorescence quenching properties and DNA interaction.
- Development of an optical miRNA sensor utilizing β-Ni(OH)2 nanosheets and duplex-specific nuclease signal amplification.
Main Results:
- β-Ni(OH)2 nanosheets exhibit high fluorescence quenching ability and differential affinity for single- and double-stranded DNA.
- Absorption properties are tunable by altering cations, pH, and DNA length.
- A sensitive optical miRNA sensor with a 1 pM detection limit was successfully developed.
- The sensor demonstrated effective miRNA analysis in cancer cell samples.
Conclusions:
- β-Ni(OH)2 nanosheets provide a versatile and effective platform for developing fluorescent biosensors.
- The developed optical miRNA sensor shows great potential for clinical diagnosis and multiplexed analysis.
