Related Experiment Videos
In vivo generation of R68.45-pPGH1 hybrid plasmids conferring a Phl+ (meta pathway) phenotype
H Herrmann1, D Janke, S Krejsa
1Sektion Biologie, Ernst-Moritz-Arndt-Universität, DDR, Greifswald.
Molecular & General Genetics : MGG
|September 1, 1988
Abstract:
Plasmid pPGH1 originating from Pseudomonas putida strain H carries all the genes required for the degradation of phenol (or cresols) via the meta cleavage pathway. Besides mobilization of pPGH1 by a plasmid of the incompatibility group P-1, hybrid plasmids conferring the Phl+ phenotype could be selected, when R68.45 was the conjugative plasmid. The hybrids contain the complete R68.45 and part of pPGH1. Integration of Phl-DNA of pPGH1 into R68.45 occurred exclusively via the IS21 region of R68.45.
Insights
Pseudomonas putida strain H
Area of Science:
- Microbiology
- Molecular Biology
- Environmental Science
Background:
- Plasmid pPGH1 from Pseudomonas putida strain H contains genes for phenol and cresol degradation.
- The meta cleavage pathway is crucial for aromatic compound breakdown.
Purpose of the Study:
- To investigate the genetic basis of phenol degradation in Pseudomonas putida.
- To explore the transfer and integration of phenol degradation genes into other plasmids.
Main Methods:
- Conjugation experiments using R68.45 as a conjugative plasmid.
- Selection and characterization of hybrid plasmids conferring the Phl+ phenotype.
Main Results:
- Hybrid plasmids were successfully created by integrating part of pPGH1 into R68.45.
- Integration of phenol degradation genes (Phl-DNA) occurred specifically via the IS21 region of R68.45.
- The resulting hybrid plasmids conferred the phenol degradation (Phl+) phenotype.
Conclusions:
- The IS21 region of R68.45 mediates the integration of foreign DNA.
- This study elucidates mechanisms for the transfer of catabolic genes between bacteria.
- Findings contribute to understanding microbial degradation of aromatic pollutants.