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Related Experiment Video

Updated: Dec 24, 2025

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
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A photostable cationic fluorophore for long-term bioimaging.

Lei Wang1, Qi Xia, Meirong Hou

  • 1School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, P. R. China. cejqqu@scut.edu.cn.

Journal of Materials Chemistry. B
|April 9, 2020
PubMed
Summary

Researchers developed a novel cationic fluorophore (PPB) for efficient long-term bioimaging. This bright and photostable probe enables extended cellular tracing and in vivo tumor monitoring, crucial for disease research.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Medical Imaging

Background:

  • Efficient long-term bioimaging is vital for understanding cellular and molecular disease processes.
  • Development of advanced fluorescent probes is essential for improved diagnostic and research capabilities.

Purpose of the Study:

  • To develop a bright and photostable cationic fluorophore for efficient long-term bioimaging.
  • To evaluate the fluorophore's properties, including fluorescence efficiency, photostability, cytotoxicity, and biocompatibility.
  • To assess the fluorophore's efficacy in long-term in vitro cellular tracing and in vivo tumor monitoring.

Main Methods:

  • Synthesis and characterization of a novel cationic fluorophore (PPB).
  • Assessment of fluorescence properties (efficiency, Stokes shift).
  • Evaluation of photostability, cytotoxicity, and biocompatibility.
  • In vitro tracing of HeLa cells over multiple passages.
  • In vivo monitoring of tumor growth.

Main Results:

  • A bright and photostable cationic fluorophore (PPB) was successfully developed.
  • PPB demonstrated high fluorescence efficiency, large Stokes shift, low cytotoxicity, and good biocompatibility.
  • PPB exhibited comparable photostability to commercial cell trackers.
  • PPB enabled tracing of HeLa cells for 16 passages in vitro and monitoring of tumor growth for 27 days in vivo.

Conclusions:

  • The developed PPB is a promising fluorescent probe for long-term bioimaging.
  • Its advanced properties make it suitable for tracking cells and monitoring disease progression over extended periods.
  • PPB offers a valuable tool for cellular and molecular research in disease studies.