HPLC Method Validation for Jatropha dioica Extracts Analysis

Area of Science:

• Pharmacognosy
• Analytical Chemistry
• Phytochemistry

Background:

• Jatropha dioica is a key plant in Mexican traditional medicine.
• Its roots exhibit antimicrobial, antiviral, and cytotoxic properties.
• Key bioactive compounds include riolozatrione and related jatrophatriones.

Purpose of the Study:

• To develop and validate an HPLC/DAD method for analyzing major compounds in Jatropha dioica extracts.
• To assess the method's performance across varying solvent polarities and seasonal collections.
• To quantify riolozatrione in Jatropha dioica samples from different years (2014-2017).

Main Methods:

• High-Performance Liquid Chromatography with Diode Array Detection (HPLC/DAD) was employed.
• An AccQ-Tag column and a water-acetonitrile gradient mobile phase were used.
• UV detection was set at 254 nm with a flow rate of 0.2 mL/min.

Main Results:

• The method demonstrated excellent reproducibility for retention times and peak areas of key compounds.
• Relative standard variations were below 4.5% for retention times and 10.5% for peak areas.
• Quantification of riolozatrione showed high accuracy (102-108% recovery) and precision (RSD < 2.5%).

Conclusions:

• The validated HPLC/DAD method is robust and suitable for analyzing Jatropha dioica extracts.
• Solvent polarity did not impact the chromatographic method's performance.
• The method enables reliable quantification of riolozatrione in Jatropha dioica across different seasons and years.