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A novel method to quantify cutaneous vascular innervation.

Eunhee Sohn1, Bum Chun Suh2, Ningshan Wang3

  • 1Department of Neurology, Chungnam University Hospital, Daejeon, South Korea.

Muscle & Nerve
|April 10, 2020
PubMed
Summary
This summary is machine-generated.

A new stereological method accurately quantifies skin nerve and blood vessel density. This method revealed lower neurovascular density in diabetes patients, correlating with autonomic dysfunction.

Keywords:
capillaryneuropathyneurovascularskin biopsyvascular

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Area of Science:

  • Neuroscience
  • Diabetology
  • Histology

Background:

  • Diabetes mellitus is associated with microvascular and neurological complications.
  • Quantifying cutaneous neurovascular changes in diabetes requires precise methodologies.
  • Existing methods may lack the accuracy needed for detailed analysis of nerve and vessel density.

Purpose of the Study:

  • To develop and validate an unbiased stereological method for quantifying cutaneous nerve, blood vessel, and neurovascular contact density.
  • To compare these densities between individuals with diabetes and healthy controls.
  • To assess the correlation of these densities with clinical and autonomic function measures.

Main Methods:

  • Skin biopsies were obtained from patients with diabetes (n=20) and control subjects (n=19).
  • Dual immunofluorescent staining was employed to visualize nerves and blood vessels.
  • Unbiased stereology was used for quantitative analysis of nerve, vessel, and neurovascular densities.

Main Results:

  • The developed stereological methods demonstrated high inter-rater and intra-rater reliability (>0.85).
  • Patients with diabetes exhibited significantly lower nerve density (P<0.05), vessel density (P<0.01), and neurovascular density (P<0.01) compared to controls.
  • Quantified densities correlated significantly with autonomic function, clinical examination findings, and symptom scores.

Conclusions:

  • An unbiased stereological approach provides a reliable method for quantifying cutaneous neurovascular parameters.
  • Reduced cutaneous nerve, vessel, and neurovascular densities are characteristic of diabetes.
  • This method offers new insights into the pathophysiology of cutaneous neurovascular changes in diabetes and other diseases.