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Compounds that disrupt proton motive force can block natural competence, stopping the spread of antibiotic resistance between bacteria. This discovery offers a potential strategy to minimize antibiotic resistance in clinical settings.

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Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Horizontal gene transfer (HGT) is a primary mechanism for the dissemination of antibiotic resistance genes among bacteria.
  • Natural competence (COM) is a key process enabling bacteria to acquire free DNA from their environment, facilitating HGT.

Purpose of the Study:

  • To investigate whether targeting cellular energy processes can inhibit natural competence and subsequent HGT of antibiotic resistance.

Main Methods:

  • Utilized compounds known to disrupt the proton motive force (PMF) in bacterial cells.
  • Assessed the impact of these compounds on bacterial natural competence.
  • Quantified the inhibition of intraspecies HGT and the exchange of antibiotic resistance genes.

Main Results:

  • Compounds disrupting proton motive force effectively blocked natural competence in bacteria.
  • This blockade significantly interrupted intraspecies HGT and the transfer of antibiotic resistance elements.
  • Demonstrated a direct link between PMF and the ability of bacteria to undergo COM.

Conclusions:

  • Disrupting the proton motive force is a viable strategy to inhibit bacterial natural competence.
  • Interfering with COM can halt the spread of antibiotic resistance via HGT.
  • This approach holds promise for developing novel strategies to combat the clinical spread of antibiotic resistance.