Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

44.3K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
44.3K
DNA Isolation01:34

DNA Isolation

198.7K
DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.
198.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Global population structures and demographic history of Suillus luteus, a pine co-introduced ectomycorrhizal fungus associated with exotic forestry and invasion.

The New phytologist·2026
Same author

Multiple hypervariable markers improve mycobiome classification in metatranscriptome and metagenome data.

Communications biology·2026
Same author

A Multi-Year Monitoring of Swiss Grain Maize: Which Cropping Factors Influence <i>Fusarium</i> Species Incidence and Associated Mycotoxins?

Toxins·2026
Same author

Global population structures and demographic history of <i>Suillus luteus</i> , a pine co-introduced ectomycorrhizal fungus associated with exotic forestry and invasion.

bioRxiv : the preprint server for biology·2026
Same author

Autologous Hematopoietic Stem Cell Transplantation for Paraneoplastic Cerebellar Degeneration.

Neurology(R) neuroimmunology & neuroinflammation·2025
Same author

A novel technique utilizing enriched <sup>15</sup>N<sub>2</sub> to trace nitrogen transfer in grass and legume mixtures.

Scientific reports·2025
Same journal

Correction: Peptine et al. Methicillin-Resistant <i>Staphylococcus aureus</i> (MRSA) and Vancomycin-Resistant Enterococci (VRE) in Nosocomial Infections: A Systematic Review of Resistance, Pathogenesis, and Clinical Management. <i>Microorganisms</i> 2026, <i>14</i>, 428.

Microorganisms·2026
Same journal

Torque Teno Virus (TTV) Plasma Load and Immune Reconstitution in People Living with HIV: A Systematic Review.

Microorganisms·2026
Same journal

Optimizing Bacteriophage Screening and Isolation Methods for Microbial Samples Derived from Different Body Sites of Cattle.

Microorganisms·2026
Same journal

Enhanced Biphenyl Degradation by <i>Rhodococcus</i> sp. TG-1 Under Cr(VI) Stress via Modified Biochar Immobilization.

Microorganisms·2026
Same journal

In Vitro Detection of Biologically Active Staphylococcal Enterotoxins Type B and C1 as an Alternative to In Vivo Testing.

Microorganisms·2026
Same journal

Monitoring Hygiene Protocols and Exploring Alternatives to Counteract Resistant Pathogens: A Case Study from Southern Italy on Healthcare-Associated Infection Control.

Microorganisms·2026
See all related articles

Related Experiment Video

Updated: Dec 24, 2025

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
10:03

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Published on: August 26, 2016

32.1K

Improved Protocol for DNA Extraction from Subsoils Using Phosphate Lysis Buffer.

Victor Guerra1, Lukas Beule2, Ena Lehtsaar2

  • 1North Florida Research and Education Center, Soil and Water Sciences Department, University of Florida, Quincy, FL 32351, USA.

Microorganisms
|April 11, 2020
PubMed
Summary
This summary is machine-generated.

A new DNA extraction method effectively isolates microbial genetic material from challenging subsoils. This cost-effective protocol enhances soil biology research by overcoming clay-induced DNA binding issues.

Keywords:
clayey soilsinter-laboratory comparisonsoil DNA extractionsoil bacteriasoil fungisubsoil

More Related Videos

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
11:24

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Published on: November 10, 2009

26.3K
Assaying for Inorganic Polyphosphate in Bacteria
07:20

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

9.1K

Related Experiment Videos

Last Updated: Dec 24, 2025

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils
10:03

Extraction and Analysis of Microbial Phospholipid Fatty Acids in Soils

Published on: August 26, 2016

32.1K
Extraction of High Molecular Weight Genomic DNA from Soils and Sediments
11:24

Extraction of High Molecular Weight Genomic DNA from Soils and Sediments

Published on: November 10, 2009

26.3K
Assaying for Inorganic Polyphosphate in Bacteria
07:20

Assaying for Inorganic Polyphosphate in Bacteria

Published on: January 21, 2019

9.1K

Area of Science:

  • Soil Science
  • Microbiology
  • Molecular Biology

Background:

  • Subsoil microbial communities are crucial for ecosystem functions.
  • High clay content in subsoils impedes DNA extraction due to nucleic acid binding.
  • Efficient DNA recovery is essential for understanding subsoil biology.

Purpose of the Study:

  • To develop an efficient and reproducible DNA extraction method for subsoils.
  • To optimize lysis and washing steps for maximal DNA yield from clay-rich soils.
  • To validate the new method against existing techniques and across diverse subsoil types.

Main Methods:

  • Development of a novel DNA extraction protocol using sodium dodecyl sulfate (SDS) lysis and phosphate buffer.
  • Optimization of bead beating, incubation temperature, and buffer concentrations.
  • Quantification of bacterial 16S and fungal 18S rRNA genes to assess DNA yield.
  • Comparison with the cetyltrimethylammonium bromide (CTAB) method and validation on multiple subsoil samples.
  • Interlaboratory comparison to confirm reproducibility and robustness.

Main Results:

  • The optimized protocol involves 1 minute of bead beating and 10 minutes of incubation at 65°C with 1 M phosphate buffer and 0.5% SDS.
  • This method significantly improved DNA recovery from clay-rich subsoils compared to the CTAB method.
  • The protocol demonstrated high reproducibility and robustness across different subsoil types and in interlaboratory tests.
  • Successful amplification of bacterial 16S and fungal 18S rRNA genes confirmed the quality of extracted DNA.

Conclusions:

  • A cost-effective and reproducible DNA extraction protocol was successfully developed for subsoil samples.
  • This method addresses the challenge of low DNA yield in clay-rich subsoils.
  • The optimized protocol provides a valuable tool for advancing DNA-based research in subsoil microbiology and ecology.