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Design of a Multiplexed Analyte Biosensor using Digital Barcoded Particles and Impedance Spectroscopy
Shreya Prakash1, Brandon K Ashley2, Patrick S Doyle3
1Department of Electrical and Computer Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.
Scientific Reports
|April 11, 2020
Summary
This study introduces digital barcoded particles and microfluidic impedance detection for cost-effective multiplexed analyte quantification. This novel approach offers a simpler, more affordable alternative to flow cytometry for disease diagnostics.
Area of Science:
- Biomedical Engineering
- Microfluidics
- Analytical Chemistry
Background:
- Multiplexing assays offer efficiency gains over individual tests but current gold standards like flow cytometry are costly and complex.
- There is a need for accessible, cost-effective multiplexing technologies in biomedical diagnostics.
Purpose of the Study:
- To design and model a novel microfluidic system for multiplexed analyte quantification using digital barcoded particles.
- To develop a non-fluorescence-based impedance detection method for high-throughput screening.
Main Methods:
- Simulation and modeling of microfluidic impedance detection using digitally barcoded polymer microparticles.
- Design of barcoded particles with specific coding regions for distinct digital barcoding patterns.
- Evaluation of electrical signatures based on adhered microsphere position and orientation.
Main Results:
- Demonstrated a 7 µm microsphere limit of detection using electrical signatures.
- Successfully enumerated micron-sized spheres in a single assay using various barcoded particle configurations.
- Developed a digital barcoding system enabling numerous distinct patterns for multiplexing.
Conclusions:
- The proposed microfluidic system provides a simplified and cost-effective method for multiplexed analyte quantification.
- This technology has potential applications in diagnosing and managing critical care diseases such as sepsis, kidney injury, UTIs, and HIV/AIDS.
- The digital barcoded particles and impedance detection offer a promising alternative to existing complex multiplexing platforms.
