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[Classes of rat lymphocyte surface immunoglobulins detected by enzymatic radioiodination]
By means of enzymatic radioiodination, immunopre-cipitation and acrylamide gel electrophoresis in sodium dodecyl sulfate it was found that on the surface of normal rat splenocytes there were two main immunoglobulin classes--monomeric IgM and Ig (H2L2) which had a heavy chain larger than they phi, yet smallerthan the micron chain, differing from them by the antigenic properties. It is possible that this Ig class corresponds to the human IgD and the mouse IgD-like cell surface molecules. Small amounts of thedetected cell surface IgG may indicate both its cytophilic properties and the immunological status of the experimental animals.
By means of enzymatic radioiodination, immunopre-cipitation and acrylamide gel electrophoresis in sodium dodecyl sulfate it was found that on the surface of normal rat splenocytes there were two main immunoglobulin classes--monomeric IgM and Ig (H2L2) which had a heavy chain larger than they phi, yet smallerthan the micron chain, differing from them by the antigenic properties. It is possible that this Ig class corresponds to the human IgD and the mouse IgD-like cell surface molecules. Small amounts of thedetected cell surface IgG may indicate both its cytophilic properties and the immunological status of the experimental animals.