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Rapid Yeast Cell Viability Analysis by Using a Portable Microscope Based on the Fiber Optic Array and Simple Image

Weiming Wang1,2, Hang Liu1,2, Yan Yu1,2

  • 1Faculty of Electronic Information and Electrical Engineering, Dalian University of Technology, No. 2 Linggong Road, Ganjingzi District, Dalian 116024, China.

Sensors (Basel, Switzerland)
|April 12, 2020
PubMed
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This summary is machine-generated.

Fiber optic arrays (FOA) offer a lens alternative for microscopes, demonstrating high resolution and field-of-view. FOA microscopes accurately identified live and dead yeast cells, showing promise for portable imaging applications.

Area of Science:

  • Optics and Photonics
  • Biomedical Imaging
  • Microscopy Technology

Background:

  • Traditional microscopes often rely on lenses, which can be bulky and prone to distortion.
  • Fiber optic arrays (FOA) present a novel alternative, offering potential advantages in magnification, coupling efficiency, and reduced distortion.

Purpose of the Study:

  • To demonstrate the resolution and field-of-view (FOV) of a microscope utilizing a fiber optic array.
  • To validate the imaging capabilities of FOA microscopes through biological sample analysis (yeast activity and concentration).
  • To compare the performance of FOA-based portable microscopes against traditional lens-based systems.

Main Methods:

  • Characterization of resolution and FOV for the FOA microscope.
  • Application of simple image processing techniques to analyze yeast activity and concentration.
Keywords:
FOAlensportable microscopeyeast activityyeast concentration

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  • Comparative performance analysis between FOA and lens-based portable microscopes.
  • Main Results:

    • The FOA microscope demonstrated significant resolution and FOV capabilities.
    • Accurate identification of live (92.1%) and dead (84.8%) yeast cells was achieved via image processing, excluding clusters.
    • Performance comparison indicated factors influencing FOA microscope imaging quality.

    Conclusions:

    • Fiber optic arrays are a viable alternative or supplement to lenses in microscopy.
    • FOA microscopes show potential for accurate biological sample analysis and portable imaging.
    • Further analysis of factors affecting FOA imaging performance is warranted.