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Updated: Dec 24, 2025

Efficient Agroinfiltration of Plants for High-level Transient Expression of Recombinant Proteins
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Biolistic Approach for Transient Gene Expression Studies in Plants.

Benoît Lacroix1, Vitaly Citovsky2

  • 1Department of Biochemistry and Cell Biology, State University of New York, Stony Brook, NY, USA. benoit.lacroix@stonybrook.edu.

Methods in Molecular Biology (Clifton, N.J.)
|April 12, 2020
PubMed
Summary

Microparticle bombardment, or biolistics, is a key technique for delivering nucleic acids into plant cells. This method is crucial for plant research, enabling gene expression studies and genome editing.

Keywords:
BiolisticMicrobombardmentMicroparticle bombardmentNucleic acid deliveryTransient gene expression

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Biotechnology

Background:

  • Biolistic or microparticle bombardment has been a vital technique since the late 1980s.
  • It facilitates the delivery of exogenous nucleic acids into living plant cells.

Purpose of the Study:

  • To review the technical aspects of the biolistic method.
  • To highlight major applications in plant research, focusing on transient gene expression.

Main Methods:

  • High-velocity microprojection of nucleic acid-coated particles into plant cells.
  • Application of the biolistic method for various molecular and genetic studies.

Main Results:

  • Demonstrated utility in transient gene expression studies.
  • Successful application in diverse research areas including gene expression, protein localization, virology, gene silencing, and genome editing.

Conclusions:

  • The biolistic method remains an invaluable tool for plant science.
  • Its versatility supports fundamental research and agricultural applications, including advanced genome editing techniques.