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Experimental mumps virus-induced labyrinthitis. Immunohistochemical and ultrastructural studies
K Tanaka1, S Fukuda, T Suenaga
1Department of Otolaryngology, Hokkaido University School of Medicine, Sapporo, Japan.
Acta Oto-Laryngologica. Supplementum
|January 1, 1988
Summary
Mumps virus infection in guinea pigs primarily affected the cochlea, causing severe organ of Corti degeneration after direct inner ear inoculation. Viral antigens were found in the stria vascularis and Reissner's membrane.
Area of Science:
- Virology
- Otolaryngology
- Pathology
Background:
- Mumps virus is a known human pathogen.
- Inner ear infections can lead to hearing loss and vestibular dysfunction.
Purpose of the Study:
- To investigate the effects of mumps virus (Torii strain) infection on the guinea pig cochlea.
- To identify the specific locations of viral replication and associated pathological changes within the inner ear.
Main Methods:
- Guinea pigs were inoculated with mumps virus via intralabyrinthine or intravascular routes.
- Cochlear tissues were examined using immunofluorescence and electron microscopy.
Main Results:
- Intralabyrinthine inoculation resulted in detectable viral infection in the cochlea, predominantly in the stria vascularis.
- Severe degeneration of the organ of Corti, particularly in the basal turn, was a common finding.
- Morphological evidence of viral replication, including nucleocapsids and budding virions, was observed in the stria vascularis and Reissner's membrane.
Conclusions:
- Direct intralabyrinthine inoculation is an effective method for inducing mumps virus infection in the guinea pig cochlea.
- Mumps virus infection can lead to significant cochlear pathology, including damage to the organ of Corti.
- The distribution of viral antigen in the cochlea aligns with previous findings in other animal models.