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Determining the optimum model parameters for oligosaccharide production efficiency using response surface integrated

Abdullah Tahir Şensoy1, Hümeyra İspirli2, Enes Dertli3

  • 1Department of Biomedical Engineering, Engineering Faculty, Samsun University, Samsun, Turkey.

Preparative Biochemistry & Biotechnology
|April 14, 2020
PubMed
Summary

Glucansucrases (GTFs) produce oligosaccharides using sucrose and maltose. Optimal enzyme concentration and acceptor:donor ratio were determined using RSM and PSO for enhanced yield.

Keywords:
Central composite designoligosaccharide production efficiencyparticle swarm optimizationresponse surface method

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Area of Science:

  • Biochemistry
  • Enzymology
  • Carbohydrate Chemistry

Background:

  • Glucansucrases (GTFs) synthesize α-glucans from sucrose, transferring glucosyl units to acceptor molecules.
  • Acceptor reactions are influenced by various parameters, impacting oligosaccharide production.

Purpose of the Study:

  • To optimize reaction parameters for producing glucansucrase-based oligosaccharides.
  • To investigate the roles of enzyme concentration, acceptor:donor ratio, and reaction time.

Main Methods:

  • Utilized a hybrid approach combining Response Surface Methodology (RSM) and Particle Swarm Optimization (PSO).
  • Employed Central Composite Design for experimental design and parameter testing.
  • Validated PSO results through experimental verification.

Main Results:

  • Enzyme concentration was identified as the most critical parameter for oligosaccharide yield.
  • Optimal enzyme concentration was determined to be 3.45 U, with an optimal acceptor:donor ratio of 0.62.
  • Experimental validation confirmed the accuracy of the PSO-derived optimal parameters.

Conclusions:

  • Enzyme concentration and acceptor:donor ratio significantly influence oligosaccharide production by GTFs.
  • The combined RSM-PSO approach effectively optimizes GTF acceptor reactions.
  • Findings enhance understanding of GTF enzymatic mechanisms and oligosaccharide synthesis.