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miR-375 Inhibits Autophagy and Further Promotes Inflammation and Apoptosis of Acinar Cells by Targeting ATG7

Shang-Ping Zhao1, Can Yu, Kai-Min Xiang

  • 1From the Department of Intensive Care Unit, The Third Xiangya Hospital of Central South University, Changsha, China.

Pancreas
|April 14, 2020
PubMed
Abstract

Insights

MicroRNA-375 (miR-375) promotes severe acute pancreatitis (SAP) by inhibiting autophagy via ATG7, increasing inflammation and cell apoptosis. This study clarifies miR-375

Area of Science:

  • Molecular Biology
  • Gastroenterology
  • Cell Biology

Background:

  • MicroRNAs (miRNAs) are implicated in severe acute pancreatitis (SAP) development.
  • MicroRNA-375 (miR-375) is a potential biomarker for SAP.

Purpose of the Study:

  • To investigate the regulatory role of miR-375 in SAP.
  • To elucidate the mechanism by which miR-375 influences SAP progression.

Main Methods:

  • SAP models were established using cerulein and lipopolysaccharide in vivo and AR42J cells in vitro.
  • Apoptosis was assessed by flow cytometry.
  • Inflammatory factors, ATG7 expression, and autophagy were analyzed using ELISA, qRT-PCR, and immunohistochemistry.
  • Pathological changes were observed via H&E staining.

Main Results:

  • miR-375 levels were upregulated in pancreatitis tissues and cell lines.
  • Overexpression of miR-375 enhanced acinar cell apoptosis and inflammation by inhibiting autophagy.
  • miR-375 directly targets and regulates ATG7, suppressing autophagy.

Conclusions:

  • miR-375 inhibits autophagy and promotes inflammation and apoptosis in SAP by targeting ATG7.
  • This study demonstrates that miR-375 modulates SAP development through ATG7 regulation.

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