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LncRNA SOX2OT affects cervical cancer cell growth, migration and invasion by regulating SOX2

Xiaohan Chang1, Huijie Zhang1, Qing Yang1

  • 1Department of Obstetrics and Gynecology, Shengjing Hospital of China Medical University , Shenyang, People's Republic of China.

Cell Cycle (Georgetown, Tex.)
|April 15, 2020
PubMed

Insights

Long non-coding RNA SOX2 overlapping transcript (SOX2OT), specifically SOX2OT-7, promotes cervical cancer growth and spread by regulating SOX2. This lncRNA is a potential biomarker for both HPV-negative and HPV-positive cervical cancers.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Long non-coding RNAs (lncRNAs) are increasingly recognized for their roles in cancer.
  • SOX2 overlapping transcript (SOX2OT) is implicated in various cancers, but its function in cervical cancer is unclear.
  • SOX2OT harbors the SOX2 gene, suggesting a potential regulatory relationship.

Purpose of the Study:

  • To investigate the expression patterns of SOX2OT variants in cervical cancer.
  • To elucidate the biological function of SOX2OT in cervical cancer progression.
  • To determine the relationship between SOX2OT and SOX2 in cervical cancer.

Main Methods:

  • Screening of SOX2OT transcript variants in cervical cancer cell lines (C-33A and SiHa).
  • Functional assays including cell viability, cell cycle, migration, and invasion assays after SOX2OT manipulation.
  • Rescue experiments to confirm SOX2's role in SOX2OT-mediated effects.
  • Analysis of SOX2 expression levels and protein stability.

Main Results:

  • SOX2OT transcript 7 (SOX2OT-7) and SOX2 were highly expressed in both HPV-negative (C-33A) and HPV16-positive (SiHa) cervical cancer cells.
  • Knockdown of SOX2OT suppressed cell viability, arrested cell cycle, and reduced migration and invasion.
  • Overexpression of SOX2OT-7 enhanced proliferation, migration, and invasion.
  • SOX2OT positively regulated SOX2 expression and protein stability.
  • Inhibition of SOX2 counteracted the malignant phenotypes induced by SOX2OT-7.

Conclusions:

  • lncRNA SOX2OT promotes cervical cancer cell growth, migration, and invasion by modulating SOX2.
  • SOX2OT-7 is identified as a potential diagnostic biomarker for both HPV-negative and HPV16-positive cervical cancers.
Keywords:
Cervical cancerSOX2SOX2OTlncRNAtranscript variants

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