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Updated: Dec 24, 2025

MicroRNA Amplification and Recognition through Locked-nucleic-acid In situ Hybridization as A Novel Detection and Quantification Method
Published on: October 7, 2025
Homogeneous multiplexed digital detection of microRNA with ligation-rolling circle amplification
Zhian Hu1, Fujian Xu1, Gongwei Sun1
1Department of Chemistry, Tsinghua University, Beijing 100084, China. sczhang@mail.tsinghua.edu.cn.
Abstract:
A simple multiplexed digital microRNA detection strategy with fluorescence flow cytometry was proposed. By isothermal ligation-rolling circle amplification, multiplexed microRNAs could be simultaneously converted to a series of nanoflower balls (NFBs) and counted by flow cytometry directly.
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