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Asymmetric Lipid Bilayer

Asymmetric Lipid Bilayer

Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...

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Updated: Dec 24, 2025

Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

Antigens Protected Functional Red Blood Cells By The Membrane Grafting Of Compact Hyperbranched Polyglycerols

Published on: January 2, 2013

Hybrid Erythrocyte Liposomes: Functionalized Red Blood Cell Membranes for Molecule Encapsulation.

Sebastian Himbert^1,2, Matthew J Blacker^1,2, Alexander Kihm^1,2,3

¹Department of Physics and Astronomy, McMaster University, Hamilton, ON, L8S 4M1, Canada.

Advanced Biosystems

|April 16, 2020

Summary

Researchers created hybrid erythrocyte liposomes by doping red blood cell membranes with synthetic lipids. These novel liposomes show tunable properties for advanced drug delivery and biomedical applications.

Keywords:

drug delivery erythrocyte membrane hybrid erythrocyte membranes membrane material properties red blood cells synthetic lipids

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Published on: March 9, 2013

Area of Science:

Biomaterials Science
Lipid Nanotechnology
Drug Delivery Systems

Background:

Erythrocyte membrane modification offers potential for enhanced drug delivery.
Developing hybrid liposomes combines natural cell membranes with synthetic lipids.

Purpose of the Study:

To fabricate and characterize hybrid erythrocyte liposomes by doping red blood cell membranes with synthetic lipids.
To investigate the impact of lipid composition and saturation on hybrid liposome properties.
To demonstrate the utility of these hybrid liposomes for small molecule encapsulation.

Main Methods:

Fabrication of hybrid erythrocyte liposomes using various synthetic lipids (PC, PS, PG) with different saturation.
Characterization using X-ray diffraction, epi-fluorescent microscopy, dynamic light scattering (DLS), Zeta potential, UV-vis spectroscopy, and Molecular Dynamics (MD) simulations.
Demonstration of small molecule encapsulation using fluorescein labeled dextran.

Main Results:

Solubility limits of synthetic lipids in erythrocyte membranes were determined.
Membrane thickness and lipid orientation were tunable by incorporating phosphatidylcholine lipids.
Hybrid membranes could be fluorescently labeled and their charge modified using specific lipids.
Successful encapsulation of small molecules (fluorescein labeled dextran) was achieved.

Conclusions:

Hybrid erythrocyte liposomes can be successfully fabricated with tunable material properties.
These hybrid liposomes represent a promising platform for drug delivery and biomedical applications.
The ability to modify membrane properties and encapsulate molecules highlights their versatility.