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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Updated: Dec 24, 2025

Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation
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Author Spotlight: Preservation of Bioenergetic Parameters in Peripheral Blood Mononuclear Cells After Cryopreservation

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Paper-Based Cell Cryopreservation.

Roaa Alnemari1, Pavithra Sukumar1, Muhammedin Deliorman1

  • 1Division of Engineering, New York University Abu Dhabi (NYUAD), Abu Dhabi, 129188, UAE.

Advanced Biosystems
|April 16, 2020
PubMed
Summary
This summary is machine-generated.

A novel paper-based method offers simple, cost-effective cell cryopreservation. This technique ensures efficient storage and retrieval, comparable to traditional methods, and supports 3D cell cultures.

Keywords:
3D culturecryopreservationmammalian cellspaperspheroids

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Materials Science

Background:

  • Efficient cell cryopreservation is crucial for various sectors, including cell storage, transport, and research.
  • Current methods often require specialized equipment and complex procedures, highlighting the need for simpler alternatives.
  • A unified platform for easy cell storage and retrieval is still lacking.

Purpose of the Study:

  • To introduce a novel, paper-based cell cryopreservation method as a practical alternative to conventional techniques.
  • To evaluate the efficiency, cost-effectiveness, and ease of management of the paper-based method.
  • To assess the viability and recovery of cells cryopreserved using this new approach, including 3D cultures.

Main Methods:

  • Development of a paper-based platform for cell cryopreservation.
  • Investigation of fibronectin treatment to enhance cell release post-thawing.
  • Assessment of cell viability and recovery rates after cryopreservation and thawing.
  • Application of the method to preformed paper-based 3D cell cultures.

Main Results:

  • The paper-based method is space-saving, cost-effective, and simple to manage, requiring no modifications to standard freezing/thawing protocols.
  • Fibronectin treatment significantly improved the release of viable cells compared to untreated paper.
  • Cryopreserved cells retained viability, and remaining cells formed spheroid-like structures.
  • The method successfully cryopreserved preformed 3D cell cultures.

Conclusions:

  • The developed paper-based cryopreservation technique offers a viable, efficient, and user-friendly alternative to conventional methods.
  • Fibronectin treatment is beneficial for enhancing cell recovery from the paper matrix.
  • This method shows promise for the cryopreservation of both individual cells and 3D cell cultures, simplifying storage and transport.