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Related Concept Videos

Retroviruses02:33

Retroviruses

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Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
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Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

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As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
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LTR Retrotransposons03:08

LTR Retrotransposons

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LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
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Related Experiment Video

Updated: Dec 24, 2025

Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins
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Simplified Reverse Genetics Method to Recover Recombinant Rotaviruses Expressing Reporter Proteins

Published on: April 17, 2020

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Reverse genetics system for human rotaviruses.

Satoshi Komoto1, Saori Fukuda1, Takayuki Murata1

  • 1Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan.

Microbiology and Immunology
|April 16, 2020
PubMed
Summary
This summary is machine-generated.

Reverse genetics technology enables virus biology study and vaccine development. A new human rotavirus (HuRVA) reverse genetics system has now been established, allowing for the creation of infectious HuRVAs from complementary DNA (cDNA).

Keywords:
11-plasmid systemNSP2NSP5T7 plasmidshuman rotavirusreverse genetics

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Area of Science:

  • Virology
  • Molecular Biology
  • Vaccinology

Background:

  • Reverse genetics is crucial for understanding virus biology and developing viral vectors and vaccines.
  • Human rotaviruses (HuRVA) have historically been challenging to manipulate using reverse genetics techniques.

Purpose of the Study:

  • To detail the development of a novel reverse genetics system for human rotaviruses (HuRVA).
  • To enable the generation of infectious HuRVA entirely from complementary DNA (cDNA) templates.

Main Methods:

  • Development and application of a novel reverse genetics system tailored for human rotaviruses (HuRVA).
  • Utilized cloned complementary DNA (cDNA) as the starting material for generating infectious viruses.

Main Results:

  • Successfully established a functional reverse genetics system for human rotaviruses (HuRVA).
  • Generated replicative HuRVAs derived entirely from cloned complementary DNA (cDNA) sequences.

Conclusions:

  • The newly developed HuRVA reverse genetics system overcomes previous technological limitations.
  • This advancement facilitates the study of rotavirus biology and the creation of potential vaccines and viral vectors.