Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Estimating long-term care needs in data-scarce settings: a diagnostic model with evidence from MENA.

Population health metrics·2026
Same author

Treatment Algorithm for Patients with Obesity in India: A Joint Consensus by Endocrine Society of India and Obesity Surgeons Society of India.

Obesity surgery·2026
Same author

Impact of Non-Malignant Portal Vein Thrombosis in Recipients with Metabolic Dysfunction-Associated Steatotic Liver Disease Compared to Other Transplant Indications.

Journal of clinical medicine·2026
Same author

Cyclopeptide-Based Fluorescent Conjugates for Monitoring Prefibrillar Aβ Nanostructures.

Journal of medicinal chemistry·2026
Same author

Molecular Networking-Driven Discovery of Carapins A-H, Bioactive Limonoids from <i>Carapa macrantha</i> Harms (Meliaceae).

Journal of natural products·2026
Same author

Peptidomimetics Inspired by α-Synuclein or Its Chaperone αB-Crystallin Differentially Modulate α-Synuclein Aggregation.

Journal of medicinal chemistry·2026

Related Experiment Video

Updated: Dec 24, 2025

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.7K

Phenothiazine-Biaryl-Containing Fluorescent RGD Peptides.

Elmira Ghabraie1, Isabell Kemker1, Nicolo Tonali1

  • 1Department of Chemistry, Organic and Bioorganic Chemistry, Bielefeld University, PO Box 100131, 33501, Bielefeld, Germany.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|April 17, 2020
PubMed
Summary

Researchers developed novel fluorescent cyclic RGD peptides by using Suzuki-Miyaura cross-coupling. These compounds target integrin αVβ3, a key protein in cancer, offering potential for new diagnostic and therapeutic applications.

Keywords:
cross-coupling reactionscyclopeptidesfluorescent peptideintegrinphenothiazine

More Related Videos

Preparing a 68Ga-labeled Arginine Glycine Aspartate RGD-peptide for Angiogenesis
07:48

Preparing a 68Ga-labeled Arginine Glycine Aspartate RGD-peptide for Angiogenesis

Published on: January 7, 2019

7.3K
Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells
12:38

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Published on: November 6, 2021

3.0K

Related Experiment Videos

Last Updated: Dec 24, 2025

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
16:16

Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

Published on: September 13, 2013

15.7K
Preparing a 68Ga-labeled Arginine Glycine Aspartate RGD-peptide for Angiogenesis
07:48

Preparing a 68Ga-labeled Arginine Glycine Aspartate RGD-peptide for Angiogenesis

Published on: January 7, 2019

7.3K
Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells
12:38

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Published on: November 6, 2021

3.0K

Area of Science:

  • Medicinal Chemistry
  • Biochemistry
  • Organic Synthesis

Background:

  • Cyclic RGD peptides are known integrin ligands.
  • Integrins αVβ3 and α5β1 play roles in angiogenesis.
  • Integrin αVβ3 is a therapeutic target due to its presence on cancer cells.

Purpose of the Study:

  • To develop versatile synthetic strategies for fluorescent building blocks.
  • To create novel fluorescent cyclic RGD pseudopeptides.
  • To tune spectral characteristics without compromising binding affinity or selectivity.

Main Methods:

  • On-resin intramolecular Suzuki-Miyaura cross-coupling (SMC).
  • Synthesis of fluorescent cyclic RGD pseudopeptides (c(RGD(W/w)Ptz)).
  • Incorporation of phenothiazine (Ptz) boronic acid and 7-bromotryptophan (7BrTrp).

Main Results:

  • Synthesized an array of eight novel fluorescent compounds.
  • Achieved ring closure via SMC, forming a phenothiazine-indole moiety with axial chirality.
  • Identified one fluorescent compound with good affinity for integrin αVβ3.
  • Demonstrated tunable fluorescence properties based on Ptz substituents, including red spectral range emission.

Conclusions:

  • Developed a novel method for synthesizing fluorescent cyclic RGD pseudopeptides.
  • The synthesized compounds show potential as targeted agents for integrin αVβ3.
  • Tunable fluorescence properties allow for versatile applications in diagnostics and therapeutics.