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Progesterone assay by inhibition of reverse passive haemagglutination
R R Coombs1, D Kirk, J Saunders
1University Department of Pathology, Addenbrooke's Hospital, Cambridge, UK.
International Archives of Allergy and Applied Immunology
|January 1, 1988
Abstract:
An assay for the steroid hormone progesterone is described based on the inhibition of the agglutination by progesterone-bovine serum albumin conjugate of red cells coupled with monoclonal antiprogesterone antibody (reverse passive haemagglutination). A low-affinity agglutination system produced the optimal sensitivity, capable of detecting less than 1 ng/ml steroid. This assay has the simplicity and sensitivity for a potential clinical test for placental or corpus luteum function. It also serves as a model for detection of other small ligands (drugs, hormones) by inhibition of reverse passive haemagglutination.