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MicroRNA-455-5p exerts inhibitory effect in cervical carcinoma through targeting S1PR1 and blocking mTOR pathway

DongMei Hu1, ShuChun Sun2, YanWei Wang3

  • 1Department of Gynaecology and Obstetrics, DongDa Hospital of Shanxian, Shanxian, Shandong, People's Republic of China.

Archives of Gynecology and Obstetrics
|April 19, 2020
PubMed
Abstract

Insights

MicroRNA-455-5p (miR-455-5p) is downregulated in cervical cancer, inhibiting tumor growth and metastasis. It targets S1PR1, impacting cell viability and the mTOR pathway, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are crucial in cancer development.
  • MicroRNA-455-5p (miR-455-5p) dysregulation is implicated in various cancers.
  • The role of miR-455-5p in cervical carcinoma remains unexplored.

Purpose of the Study:

  • To investigate the role and mechanism of miR-455-5p in cervical carcinoma.
  • To determine the relationship between miR-455-5p and its potential target, S1PR1.
  • To elucidate the impact of miR-455-5p on cancer cell behavior and signaling pathways.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
  • MTT and Transwell assays to assess cell viability and metastasis.
  • Western blot to detect mTOR pathway activation and apoptosis.
  • Dual-luciferase reporter assay to confirm target interaction.

Main Results:

  • miR-455-5p expression is significantly decreased in cervical carcinoma, correlating with poor patient outcomes.
  • miR-455-5p suppresses cervical cancer cell viability and metastasis.
  • Sphingosine-1-phosphate receptor 1 (S1PR1) was identified as a direct target of miR-455-5p.
  • S1PR1 overexpression reversed the inhibitory effects of miR-455-5p on cell viability and metastasis.
  • miR-455-5p promotes apoptosis and inactivates the mTOR pathway in cervical cancer cells.

Conclusions:

  • miR-455-5p functions as a tumor suppressor in cervical carcinoma.
  • The miR-455-5p/S1PR1 axis plays a critical role in regulating cervical cancer progression.
  • Targeting miR-455-5p or S1PR1 may offer novel therapeutic strategies for cervical cancer.
Keywords:
Cervical carcinomaS1PR1mTOR pathwaymiR-455-5p

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