Related Experiment Video
Updated: Dec 23, 2025
05:54
Quantification of Diabetes-induced Adherent Leukocytes in Retinal Vasculature
Published on: January 24, 2025
582
Diabetes mellitus impairs circulating proangiogenic granulocytes
Roberta Cappellari1,2, Marianna D'Anna1,2, Lisa Menegazzo1,2
1Veneto Institute of Molecular Medicine, Padova, Italy.
Diabetologia
|April 20, 2020
Summary
Diabetes impairs proangiogenic granulocytes (PAGs), crucial for blood vessel formation. Improving glucose control with dapagliflozin significantly increased PAG levels and function, suggesting a therapeutic target for diabetic cardiovascular risk.
Area of Science:
- Cardiovascular Research
- Immunology
- Endocrinology
Background:
- Cardiovascular disease risk is elevated in diabetes, partly due to impaired blood vessel formation (angiogenesis).
- Diabetes negatively impacts blood cells involved in angiogenesis, including proangiogenic granulocytes (PAGs).
Purpose of the Study:
- To investigate whether diabetes impairs the function and quantity of proangiogenic granulocytes (PAGs).
- To assess the impact of glucose control on PAGs in individuals with diabetes.
Main Methods:
- Characterized and quantified CD49d+ granulocytes (PAGs) in peripheral blood from type 1/2 diabetic and control participants.
- Assessed in vitro angiogenesis assays and PAG antigenic profiles.
- Quantified PAGs before and after treatment with dapagliflozin (SGLT2 inhibitor) and in diabetic mouse models.
Main Results:
- PAGs (eosinophils/neutrophils) express CD49d, CXCR4, and VEGFR1, supporting endothelial cell angiogenesis.
- Diabetic PAGs showed significantly reduced capacity to stimulate endothelial cell tubule formation and were reduced by 30-40%.
- PAG levels increased 1.8-fold after dapagliflozin treatment, correlating inversely with plasma glucose and HbA1c.
Conclusions:
- Diabetes significantly impairs proangiogenic granulocytes (PAGs), with hyperglycemia playing a direct role.
- These findings offer mechanistic insights into defective angiogenesis in diabetes.
- Restoration of PAG function may be a therapeutic strategy for managing diabetic cardiovascular complications.
More Related Videos
08:47Live Images of GLUT4 Protein Trafficking in Mouse Primary Hypothalamic Neurons Using Deconvolution Microscopy
Published on: December 7, 2017
10.1K
07:25Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia
Published on: September 22, 2020
3.7K
Related Concept Videos
01:20Pathophysiology of Diabetes
2.9K
Diabetes mellitus is a chronic metabolic disorder characterized by hyperglycemia. The four categories of diabetes are type 1 diabetes, type 2 diabetes, other specific types of diabetes, and gestational diabetes.
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
Type 1 diabetes is characterized by autoimmune-mediated destruction of pancreatic β cells, with environmental factors potentially triggering this process in genetically susceptible individuals. Despite many not having a family history, certain genes increase susceptibility,...
2.9K
01:22Diabetes Mellitus: Overview and Type I Subtype
4.7K
Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels due to inadequate insulin production, insulin resistance, or both. The condition affects millions worldwide and can significantly impact their health and quality of life.
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
Type 1 diabetes is an autoimmune disease in which the immune system mistakenly attacks and destroys the insulin-producing beta cells in the pancreas. As a result, the body is unable to produce sufficient insulin, and individuals with...
4.7K
01:22Diabetes Mellitus: Type 2 and Gestational
4.2K
Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
4.2K
01:24Regulation of Angiogenesis and Blood Supply
3.2K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits. Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.2K
01:15Differentiation of Common Myeloid Progenitor Cells
3.8K
Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.8K
01:19Psychoneuroimmunology: Diabetes and Cancer
361
Chronic stress has been linked to both the onset and progression of serious health conditions, including Type 2 diabetes and cancer. Type 2 diabetes, a widespread chronic illness, is closely associated with obesity and insulin resistance, both of which often worsen under stress. Studies indicate that men experiencing high levels of chronic stress face a 45% higher risk of developing diabetes compared to those with minimal stress. Stress triggers physiological responses that elevate blood...
361