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Updated: Dec 23, 2025
Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
Published on: October 20, 2018
Identification of Siglec Cis-Ligands by Proximity Labeling
Amin Alborzian Deh Sheikh1, Chizuru Akatsu1, Takeshi Tsubata2
1Department of Immunology, Medical Research Institute, Tokyo Medical and Dental University, Tokyo, Japan.
Abstract:
Siglecs are known to be bound and regulated by membrane molecules that display specific sialic acid-containing ligands and are present on the same cell (cis-ligands). Because of the low-affinity binding of Siglecs to the glycan ligands, conventional methods such as immunoprecipitation are not suitable for identification of Siglec cis-ligands. Here we describe efficient and specific labeling of cis-ligands of CD22 (also known as Siglec-2) on B lymphocytes by proximity labeling using tyramide. This method may also be applicable to labeling of cis-ligands of other Siglecs.
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