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Preparation of Soluble Malectin and Its Tetramer
Sheng-Ying Qin1,2, Dan Hu3, Kazuo Yamamoto4
1Clinical Experimental Center, First Affiliated Hospital of Jinan University, Guangzhou, China. qinshengying78@163.com.
Methods in Molecular Biology (Clifton, N.J.)
|April 20, 2020
Summary
Malectin, an endoplasmic reticulum lectin, is crucial for glycoprotein quality control by recognizing specific sugar chains. Researchers prepared soluble malectin and its tetramer for detecting cell surface glycans.
Area of Science:
- Molecular Biology
- Glycobiology
- Cell Biology
Background:
- Malectin is an endoplasmic reticulum (ER)-resident lectin identified in Xenopus laevis.
- It is highly conserved across animal species and plays a role in glycoprotein quality control.
- Malectin specifically recognizes the Glc₂Man₉GlcNAc₂ (G2M9) oligosaccharide on newly synthesized glycoproteins.
Purpose of the Study:
- To describe the preparation of recombinant soluble malectin.
- To describe the preparation of recombinant soluble malectin tetramer.
- To explore the potential of these preparations as tools for detecting specific cell surface glycans.
Main Methods:
- Preparation of recombinant soluble malectin.
- Preparation of recombinant soluble malectin tetramer.
Main Results:
- Successfully prepared recombinant soluble malectin.
- Successfully prepared recombinant soluble malectin tetramer.
- These preparations show potential for detecting Glcα1-3Glc containing glycans.
Conclusions:
- Recombinant soluble malectin and its tetramer can be prepared.
- These tools may be valuable for detecting Glcα1-3Glc glycans on cell surfaces.
- Further development could enhance their utility in glycan detection.
