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Published on: June 1, 2012
Enhanced Adipose Mesenchymal Stem Cells Proliferation by Carboxymethyl-Chitosan Functionalized Polycaprolactone
Atena Shapourzadeh1, Seyed Mohammad Atyabi2, Shiva Irani3
1Department of Biochemistry, Faculty of Basic Science, Islamic Azad University Damghan Branch, Damghan, Iran.
Background:
Through combining two synthetic and natural polymers, scaffolds can be developed for tissue engineering and regenerative medicine purposes.
Methods:
In this work, carboxymethyl chitosan (CMC; 20%) was grafted to Polycaprolactone (PCL) nanofibers using the cold atmospheric plasma of helium. The PCL scaffolds were exposed to CAP, and functional groups were developed on the PCL surface.
Results:
The results of Fourier Transform Infrared Spectroscopy confirmed CMC (20%) graft on PCL scaffold. The Thiazolyl blue tetrazolium bromide assay showed a significant enhancement (p < 0.05) in the cell affinity and proliferation of adipose-derived stem cells (ADSCs) to CMC20%-graft-PCL scaffolds. After 14 days, bone differentiation was affirmed through alizarin red and calcium depositions.
Conclusion:
Based on the results, the CMC20%-graft-PCL can support the proliferation of ADSCs and induce the differentiation into bone with longer culture time.
