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Updated: Dec 23, 2025

A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction
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A Blood-based Test for the Detection of ROS1 and RET Fusion Transcripts from Circulating Ribonucleic Acid Using Digital Polymerase Chain Reaction

Published on: April 5, 2018

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A novel circular RNA circENTPD7 contributes to glioblastoma progression by targeting ROS1

Fei Zhu1, Cheng Cheng1, Hong Qin1

  • 1Department of Neuro Surgery, The Affiliated Hospital of Yangzhou University, No. 45, Taizhou Road, Yangzhou, Jiangsu China.

Cancer Cell International
|April 21, 2020
PubMed
Abstract

Insights

Circular RNAs (circRNAs) promote glioblastoma by upregulating circENTPD7. This molecule enhances tumor cell proliferation and motility by interacting with miR-101-3p and ROS1, impacting patient survival.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in human cancers, including glioblastoma.
  • Mechanisms linking circRNAs to glioblastoma pathogenesis remain largely unknown.
  • Investigating circRNA roles is crucial for understanding glioblastoma progression.

Purpose of the Study:

  • To elucidate the role of circRNAs in glioblastoma pathogenesis.
  • To identify specific circRNAs involved in glioblastoma progression.
  • To explore the molecular mechanisms underlying circRNA function in glioblastoma.

Main Methods:

  • CircRNA microarray analysis to detect dysregulated circRNAs in glioblastoma.
  • Cell proliferation assays (CCK-8) and clone formation tests to assess cellular growth.
  • Bioinformatic predictions, dual-luciferase reporter assays, and RNA-RNA pulldown assays to confirm molecular interactions.

Main Results:

  • circENTPD7 (hsa_circ_0019421) was significantly upregulated in glioblastoma tissues.
  • High circENTPD7 expression correlated with poor overall survival in glioblastoma patients.
  • Knockdown of circENTPD7 suppressed glioblastoma cell proliferation and motility.
  • circENTPD7 functions as a sponge for miR-101-3p, regulating ROS1 expression and promoting tumor growth.

Conclusions:

  • circRNA circENTPD7 promotes glioblastoma cell proliferation and motility.
  • The mechanism involves the regulation of the miR-101-3p/ROS1 axis.
  • circENTPD7 represents a potential therapeutic target for glioblastoma.
Keywords:
GlioblastomaROS1circENTPD7circRNAmiR-101-3p

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