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Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

Purification of Bionanoparticles.

L Pedro¹, S S Soares¹, G N M Ferreira¹

¹IBB-Institute for Biotechnology and Bioengineering, Centre for Molecular and Structural Biomedicine, University of Algarve, Faro, Portugal.

Chemical Engineering & Technology

|April 22, 2020

Summary

This summary is machine-generated.

Protein nanoparticles offer advantages for gene therapy but require scalable purification methods. This review examines common downstream processing techniques for efficient nanoparticle recovery and production.

Keywords:

Bionanoparticles Downstream processing Gene therapy

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Published on: June 28, 2019

Area of Science:

Biotechnology and Nanomedicine
Biopharmaceutical Engineering

Background:

Growing demand for nanoparticulate products (viruses, plasmids, protein nanoparticles, drug delivery systems) necessitates controllable production.
Protein nanoparticles are promising for gene and molecular therapy, merging benefits of viral and non-viral vectors while mitigating drawbacks.

Purpose of the Study:

To review common downstream processing methods for nanoparticle purification.
To highlight the importance of selective and scalable purification for protein nanoparticle applications.

Main Methods:

Review of existing literature on nanoparticle purification techniques.
Analysis of factors influencing downstream processing (production method, producer system, media, nanoparticle characteristics).

Main Results:

Downstream processing is critical for successful nanoparticle application in therapy.
Purification strategies are influenced by production specifics and nanoparticle structure (size, shape, architecture).

Conclusions:

Effective downstream processing is essential for the clinical translation of protein nanoparticles.
Further development of scalable and selective purification methods is needed to meet the demand for therapeutic nanoparticles.