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Types Of Column Chromatography

Types Of Column Chromatography

The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
Gel Filtration Chromatography
When the...

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Related Experiment Video

Updated: Dec 23, 2025

Author Spotlight: Improved Method for Production and Purification of Adeno-Associated Viral Vectors

Author Spotlight: Improved Method for Production and Purification of Adeno-Associated Viral Vectors

Published on: April 5, 2024

Viral clearance capacity by continuous Protein A chromatography step using Sequential MultiColumn Chromatography.

Caroline Goussen¹, Laëtitia Goldstein¹, Corinne Brèque¹

¹LFB Biotechnologies, Direction Générale du Développement, 3 avenue des Tropiques, 91940 Les Ulis, France.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences

|April 22, 2020

Summary

This summary is machine-generated.

Continuous Protein A chromatography, using Sequential MultiColumn Chromatography (SMCC) technology, effectively clears viruses in monoclonal antibody (MAb) purification. This method maintains viral safety comparable to traditional batch processing, enhancing manufacturing efficiency.

Keywords:

Continuous chromatography Monoclonal antibodies Protein A chromatography Sequential MultiColumn Chromatography (SMCC)Viral clearance

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Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow

Published on: January 8, 2014

Area of Science:

Biopharmaceutical Manufacturing
Downstream Processing
Viral Safety Evaluation

Background:

Biological protein therapeutics, like monoclonal antibodies (MAbs), require robust downstream processes for consistent quality and efficient production.
Viral safety is a critical attribute for biopharmaceuticals, necessitating thorough evaluation of viral clearance capabilities.
Protein A chromatography is a standard initial capture step for MAbs, effective in removing impurities and contributing to viral clearance.

Purpose of the Study:

To investigate the impact of batch versus multicolumn Protein A chromatography on viral clearance efficiency.
To evaluate the viral clearance performance of Novasep Sequential MultiColumn Chromatography (SMCC) technology for MAbs.
To assess viral distribution across purification cycles and columns in continuous chromatography.

Main Methods:

Small-scale Murine Minute Virus (MMV) spiking experiments were conducted.
Protein A chromatography was performed in both batch and multicolumn (SMCC) modes.
Viral reduction factors (RF) and distribution were analyzed for both methods.

Main Results:

Protein A chromatography using SMCC technology showed no statistically significant difference in viral reduction compared to batch processing (RF of 3.7 log10 vs. 3.8 log10).
Similar viral distribution was observed across purification cycles and columns in both batch and continuous modes.
The study confirmed that continuous Protein A chromatography using SMCC technology maintains efficient viral clearance capacity.

Conclusions:

Continuous Protein A chromatography with SMCC technology provides comparable viral clearance to batch processing for MAbs.
The implementation of continuous chromatography does not compromise viral safety, a critical quality attribute.
This approach offers manufacturing efficiency and flexibility while ensuring product quality and safety in biopharmaceutical production.