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Updated: Dec 23, 2025
Porcine Corneal Tissue Explant to Study the Efficacy of Herpes Simplex Virus-1 Antivirals
Published on: September 20, 2021
Lipidomic profiling of virus infection identifies mediators that resolve herpes simplex virus-induced corneal
Cuiping Zhang1, Zuojian Hu, Ke Wang
1Minhang Hospital & Institutes of Biomedical Sciences & Department of Systems Biology for Medicine, Fudan University, Shanghai, 200032, P. R. China. hongxiuyu@fudan.edu.cn pyyang@fudan.edu.cn.
Abstract:
Lipid mediators (LMs) play a pivotal role in the induction and resolution of inflammation. To identify and elucidate their involvement during virus infection, multiple reaction monitoring (MRM) based liquid chromatography-tandem mass spectrometry lipidomic profiling of 62 lipid species was performed in this study. Results show that RAW264.7 macrophages differentially produce specific LMs signals depending on difference in virus pathogenicity. Integration of large-scale lipidomics with targeted gene expression data revealed mediators, such as RVD3, 18-HEPE, 11(12)-EET etc. correlated with the pathogenic phase of the infection. The herpes simplex virus (HSV)-induced keratitis model demonstrates that 11(12)-EET treatment represents a novel alternative for treating viral infection.
