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Updated: Dec 23, 2025
Nerve Stem Cell Differentiation by a One-step Cold Atmospheric Plasma Treatment In Vitro
Published on: January 11, 2019
Inhibition of basal cell carcinoma cells by cold atmospheric plasma‑activated solution and differential gene
Xingyu Yang1, Chunjun Yang1, Liyun Wang1
1Department of Dermatology, The Second Affiliated Hospital, Anhui Medical University, Hefei, Anhui 230601, P.R. China.
Abstract:
Basal cell carcinoma is a common skin tumor. Cold atmospheric plasma (CAP) has attracted increasing attention for its antitumor effects. The aim of the present study was to investigate the effects and related mechanisms of two CAP‑activated solutions on the TE354T basal cell carcinoma and HaCat keratinocyte cell lines. Plasma‑activated solution (PAS) was prepared by CAP irradiation of DMEM and PBS. TE354T cells were treated with PAS in vitro and the effect on cell viability was evaluated by an MTT assay. The apoptosis rate was detected by Annexin V/propidium iodide staining. Furthermore, western blotting and RNA‑sequencing were performed. The present results demonstrated that PAS induced apoptotic signaling in basal cell carcinoma cells, and that this effect was associated with the activation of the MAPK signaling pathway. Therefore, the present study demonstrated that PAS may serve as a novel treatment for basal cell carcinoma.
