Phase diagram of two-patch colloids with competing anisotropic and isotropic interactions

Patchy particles are considered to be a good model for protein aggregation. We propose a novel method to generate different structures of glucose isomerase protein such as chains, crystals and bundles by utilising aggregation of two-patch colloidal particles in presence of competing isotropic and anisotropic potential. We calculate the equilibrium phase diagram of two-patch colloidal particles and demonstrates the coexistence of different phases like disordered clusters, chains, crystals and bundles depending on the relative strength of isotropic and anisotropic potential. We also show that the formation of network of bundles is metastable against the formation of thermodynamically favored finite sized bundles along with thermodynamically stable crystals. These bundles appear to be helical in structure similar to that observed in sickle cell hemoglobin. The simulation results show that the method can characterize phase behaviour of glucose isomerase protein, which provides a novel tool to unveil self-assembly mechanism of protein under different conditions.