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General Transcription Factors01:30

General Transcription Factors

6.6K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
6.6K
Transcription Factors02:16

Transcription Factors

82.0K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.0K
Combinatorial Gene Control02:33

Combinatorial Gene Control

9.4K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
9.4K
Master Transcription Regulators02:23

Master Transcription Regulators

7.6K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.6K
Master Transcription Regulators02:23

Master Transcription Regulators

2.6K
2.6K
Chromatin Modification in iPS Cells01:32

Chromatin Modification in iPS Cells

2.1K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
2.1K

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Related Experiment Video

Updated: Dec 23, 2025

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.7K

Tuning up Transcription Factors for Therapy.

Attila Becskei1

  • 1Biozentrum, University of Basel, Klingelbergstrasse 50/70, 4056 Basel, Switzerland.

Molecules (Basel, Switzerland)
|April 25, 2020
PubMed
Summary
This summary is machine-generated.

Transcription factor engineering advances therapeutic applications like cancer therapies. Understanding their efficacy principles, including LacI, TetR, TALE, and CRISPR-dCas9, aids optimization for precise gene regulation.

Keywords:
Lac repressorTAL-EffectorTet Repressoraggregationdead Cas9dissociation rate constanthomodimerizationtranscription activator-like effector

More Related Videos

Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

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Related Experiment Videos

Last Updated: Dec 23, 2025

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.7K
Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter
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Identification of Transcription Factor Regulators using Medium-Throughput Screening of Arrayed Libraries and a Dual-Luciferase-Based Reporter

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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
09:22

Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

Published on: November 26, 2013

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Synthetic Biology

Background:

  • Transcription factors (TFs) are crucial regulators of gene expression.
  • Recent advances in TF delivery and design enable therapeutic applications.
  • Efficacy and precision of TFs are paramount for successful therapeutic outcomes.

Purpose of the Study:

  • To explore the common principles governing the efficacy of prokaryotic and designer TFs.
  • To identify mechanisms for optimizing transcriptional activators and repressors for therapeutic use.
  • To guide the tailoring of natural and synthetic TFs for specific applications.

Main Methods:

  • Biophysical and genetic characterization of LacI and TetR repressors.
  • Analysis of designer transcription factors: transcription activator-like effectors (TALE) and CRISPR-dCas9.
  • Review of principles underlying TF efficacy and precision.

Main Results:

  • Common principles governing TF efficacy were revealed through characterization of LacI, TetR, TALE, and CRISPR-dCas9.
  • These principles can inform the optimization of transcriptional activators and repressors.
  • Understanding TF mechanisms is key to their therapeutic potential.

Conclusions:

  • Further research is needed on dissociation constants, enzymatic activity, phase separation, squelching, and long-range interactions.
  • Understanding these mechanisms will enable the precise tailoring of TFs for diverse applications.
  • Transcription factor engineering holds significant promise for cell replacement and cancer therapies.