Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Methods for Controlling Microbial Growth01:29

Methods for Controlling Microbial Growth

1.4K
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
1.4K
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

707
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
707
Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

906
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
906
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

843
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
843
Key Techniques in Microbiology01:19

Key Techniques in Microbiology

1.3K
Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
1.3K
Physical Methods for Controlling Microbial Growth: Temperature01:23

Physical Methods for Controlling Microbial Growth: Temperature

837
Heat is a widely used method to control microbial growth by targeting and denaturing cellular proteins, thereby killing or inactivating microbes. This method's effectiveness is quantified using parameters such as the thermal death point (TDP), thermal death time (TDT), and decimal reduction time (D value). TDP represents the lowest temperature at which all microorganisms in a liquid suspension are eliminated within 10 minutes, whereas TDT is the time necessary to achieve sterilization at a...
837

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Risks of restrictive red blood cell transfusion strategies in patients with cardiovascular disease (CVD): a meta-analysis.

Transfusion medicine (Oxford, England)·2018
Same author

International Forum regarding practices related to donor haemoglobin and iron.

Vox sanguinis·2016
Same author

CD101, a novel echinocandin with exceptional stability properties and enhanced aqueous solubility.

The Journal of antibiotics·2016
Same author

Transfusion of recently donated (fresh) red blood cells (RBCs) does not improve survival in comparison with current practice, while safety of the oldest stored units is yet to be established: a meta-analysis.

Vox sanguinis·2016
Same author

The strategies to reduce iron deficiency in blood donors randomized trial: design, enrolment and early retention.

Vox sanguinis·2014
Same author

Capillary versus venous haemoglobin determination in the assessment of healthy blood donors.

Vox sanguinis·2013
Same journal

Correlation of sample-to-cut-off ratio of anti-SARS-CoV-2 IgG antibody chemiluminescent assay with neutralization activity: a prospective multi-centric study in India.

ISBT science series·2021
Same journal

The role of affect, satisfaction and internal drive on personal moral norms during COVID-19.

ISBT science series·2021
Same journal

A prospective study on COVID-19 convalescent plasma donor (CCP) recruitment strategies in a resource constrained blood centre.

ISBT science series·2021
Same journal

Exploration of COVID-19 related fears deterring from blood donation in India.

ISBT science series·2021
Same journal

Methodological considerations for linked blood donor-component-recipient analyses in transfusion medicine research.

ISBT science series·2020
Same journal

Definition of emerging infectious diseases.

ISBT science series·2020
See all related articles

Related Experiment Video

Updated: Dec 23, 2025

Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products
04:52

Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products

Published on: July 26, 2024

2.2K

Pathogen-reduction methods: advantages and limits.

H G Klein1, B J Bryant2

  • 1Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD 20892, USA.

ISBT Science Series
|April 25, 2020
PubMed
Summary
This summary is machine-generated.

Pathogen-reduction technologies enhance blood safety by inactivating infectious agents in blood products. While effective for plasma, cellular components require further development for pathogen inactivation to prevent transfusion-transmitted infections.

Keywords:
Blood safetypathogen inactivationpathogen reductiontransfusiontransfusion safety

More Related Videos

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings
07:22

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Published on: August 19, 2021

3.3K
Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
07:47

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Published on: January 1, 2016

11.9K

Related Experiment Videos

Last Updated: Dec 23, 2025

Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products
04:52

Author Spotlight: Discovering New Biopesticides from Bioactive Soil Microbe-Derived Natural Products

Published on: July 26, 2024

2.2K
Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings
07:22

Bacteriophage Effectiveness for Biocontrol of Foodborne Pathogens Evaluated via High-Throughput Settings

Published on: August 19, 2021

3.3K
Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases
07:47

Anti-virulent Disruption of Pathogenic Biofilms using Engineered Quorum-quenching Lactonases

Published on: January 1, 2016

11.9K

Area of Science:

  • Blood safety
  • Infectious disease transmission
  • Transfusion medicine

Background:

  • Pathogen-reduction technologies offer a proactive strategy against transfusion-transmitted infections.
  • Existing technologies are successfully used in plasma fractionation, preventing viral transmission since 1987.
  • Cellular blood components cannot utilize fractionation methods, necessitating alternative pathogen-inactivation approaches.

Purpose of the Study:

  • To explore the potential of pathogen-inactivation technologies in enhancing blood product safety.
  • To address the ongoing threat of transfusion-transmitted infections from emerging or unknown pathogens.
  • To evaluate the requirements for effective pathogen-inactivation in blood components.

Main Methods:

  • Review of current pathogen-inactivation technologies and their applications.
  • Analysis of the limitations and challenges in applying these technologies to cellular blood products.
  • Assessment of criteria for acceptable pathogen-inactivation methods, including efficacy, safety, and impact on blood constituents.

Main Results:

  • Pathogen-inactivation has proven effective for plasma derivatives, eliminating transmissions of HIV, HCV, and HBV.
  • No current pathogen-inactivation technology is universally applicable to all blood components.
  • Developed technologies must ensure pathogen inactivation without compromising therapeutic efficacy or introducing toxicity.

Conclusions:

  • Pathogen-inactivation represents a crucial additional layer of protection for the global blood supply.
  • Continued research and development are essential to overcome limitations in current pathogen-inactivation technologies.
  • Implementing advanced pathogen-inactivation strategies holds significant potential to improve worldwide blood transfusion safety.