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Ano6 disruption impairs acinar cell regulatory volume decrease and protein secretion in murine submandibular salivary

Takashi Munemasa1,2, Xin Gao1, James E Melvin1

  • 1Secretory Mechanisms and Dysfunctions Section, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, Maryland.

Journal of Cellular Physiology
|April 25, 2020
PubMed
Summary

Anoctamin 6 (Ano6) is crucial for salivary gland cell volume regulation and protein secretion, particularly in females. Its absence significantly impairs regulatory volume decrease (RVD) and affects β-adrenergic-dependent protein release.

Keywords:
Ano6Tmem16fproteinregulatory volume decreasesalivary gland

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Area of Science:

  • Cell biology
  • Physiology
  • Ion channel function

Background:

  • Anoctamin 6 (Ano6) is a widely expressed calcium-dependent protein.
  • Its specific functions in salivary glands remain largely uncharacterized.
  • Ano6 is implicated in various cellular processes.

Purpose of the Study:

  • To investigate the role of Ano6 in salivary gland acinar cell function.
  • To determine Ano6's involvement in regulatory volume decrease (RVD) and secretion.
  • To explore potential sex-specific effects of Ano6 in salivary glands.

Main Methods:

  • Utilized Ano6 knockout (Ano6-/-) mouse models.
  • Assessed regulatory volume decrease (RVD) in submandibular gland (SMG) acinar cells.
  • Measured ex vivo agonist-stimulated secretion of water, ions, and proteins.

Main Results:

  • Ano6 deficiency significantly reduced RVD by approximately 70% in SMG acinar cells.
  • Ano6 is essential for the calcium-dependent RVD pathway in salivary glands.
  • Ano6 disruption did not affect water and ion secretion but reduced female-specific β-adrenergic-dependent protein secretion.

Conclusions:

  • Ano6 plays a significant role in regulating salivary gland acinar cell volume.
  • Ano6 modulates β-adrenergic-stimulated protein secretion, with a notable effect in female SMGs.
  • Ano6's function extends to both cell volume homeostasis and specific secretory pathways in salivary glands.