A safe and reusable imaging chamber compatible with organic solvents

Area of Science:

• Biomedical Engineering
• Microscopy Techniques
• Materials Science

Background:

• High refractive index organic solvents are essential for tissue clearing but pose safety risks.
• These solvents can damage sensitive microscopy equipment and harm users, particularly in shared facilities.
• Existing solutions lack adequate safety and reusability for central microscopy units.

Purpose of the Study:

• To develop a safe and reusable imaging chamber for tissue clearing microscopy.
• To mitigate risks associated with hazardous organic solvents in microscopy environments.
• To provide an accessible, downloadable design for widespread adoption.

Main Methods:

• Designed a large, reusable imaging chamber compatible with the universal mounting frame AK (PeCon GmbH).
• Focused on ease of assembly and improved user safety.
• Made the chamber design publicly available for download.

Main Results:

• The developed chamber significantly improves the working environment in central microscopy units.
• It effectively contains hazardous chemicals, protecting both users and equipment.
• The chamber is easy to assemble and reusable, offering a practical solution.

Conclusions:

• The novel imaging chamber offers a safer alternative for tissue clearing microscopy.
• This design promotes a healthier and more secure working environment in shared microscopy facilities.
• Open-access availability encourages the adoption of safer microscopy practices.