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A sensitive and rapid immunochemical method for quantitation of proteins
M R Mawal1, Y R Mawal, S N Ranadive
1Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
Bioscience Reports
|October 1, 1988
Abstract:
A sensitive and rapid ELISA for quantitation of seed globulins is described. This method employs conjugation of pigeon pea (Cajanus cajan) globulin antibodies and the enzyme peroxidase together with dextran. Using this conjugate, proteins as low as 0.1 ng were detected. Dextran conjugate has a ten-fold greater efficiency of quantitating pigeon pea globulins than the commercial goat anti-rabbit IgG conjugate, and is three-fold more efficient than pigeon pea globulin IgG peroxidase conjugate. The method can be conveniently adapted for quantitation of other proteins also.