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Long non-coding RNA AOC4P suppresses epithelial ovarian cancer metastasis by regulating epithelial-mesenchymal

Xiaojing Lin1,2,3, Xiaoyan Tang1,3, Tingting Zheng1,3

  • 1Department of Gynecology, Obstetrics and Gynecology Hospital of Fudan University, 419 Fangxie Road, Shanghai, 200011, P.R. China.

Journal of Ovarian Research
|April 27, 2020
PubMed
Abstract

Insights

Long non-coding RNA AOC4P acts as a tumor suppressor in epithelial ovarian cancer (EOC) by inhibiting metastasis. Decreased AOC4P expression correlates with advanced EOC stages and promotes epithelial-mesenchymal transition (EMT), suggesting its potential as an anti-metastatic therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • The role of long non-coding RNAs (lncRNAs) in epithelial ovarian cancer (EOC) metastasis, particularly their involvement in the epithelial-mesenchymal transition (EMT), is not well understood.
  • Identifying specific lncRNAs and their mechanisms in EOC metastasis is crucial for developing targeted therapies.

Purpose of the Study:

  • To investigate the function and mechanism of the lncRNA AOC4P in the metastasis of epithelial ovarian cancer (EOC).
  • To determine if AOC4P plays a role in the epithelial-mesenchymal transition (EMT) process within EOC.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) was used to assess AOC4P gene expression.
  • Transwell and scratch assays were employed to evaluate cell migration and invasion.
  • In vivo metastatic activity was assessed using an intraperitoneal metastasis model.
  • Downstream gene expression related to EMT was analyzed using an RT-PCR array and validated by qRT-PCR and Western blot.

Main Results:

  • AOC4P expression was found to be decreased in EOC tissues and cell lines, with lower expression correlating positively with advanced FIGO stage and lymph node metastasis.
  • Knockdown of AOC4P enhanced EOC cell migration and invasion, while its overexpression reduced metastatic ability in vitro.
  • AOC4P demonstrated an anti-metastatic role in vivo, partially mediated by the EMT process and alterations in MMP9 and COL1A2 expression.

Conclusions:

  • AOC4P plays a significant role in inhibiting EOC invasion and metastasis.
  • AOC4P has the potential to serve as a novel therapeutic target for lncRNA-based anti-metastatic strategies in EOC management.
Keywords:
AOC4PEMTMetastasisOvarian cancerlncRNA

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