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Scope and limitations of rotating-frame nuclear Overhauser enhancement spectroscopy applied to oligosaccharides
J Breg1, D Romijn, J F Vliegenthart
1Department of Bio-Organic Chemistry, University of Utrecht, The Netherlands.
Abstract:
Two-dimensional rotating-frame nuclear Overhauser enhancement spectroscopy has been applied to milk oligosaccharides containing the blood-group determinants Lea, Led, or Leb. The results indicate a large number of dipolar interactions that are consistent with the preferred conformations described for these blood-group determinants. Despite careful experimental conditions, coherent transfer processes are not suppressed completely, allowing only a qualitative evaluation of the nuclear Overhauser data. The 1H- and 13C-n.m.r. spectra are assigned by comparison with literature data and by application of two-dimensional 13C-1H shift-correlation spectroscopy.